Semiautomated metabolic staining assay for Bacillus cereus emetic toxin.
نویسندگان
چکیده
This paper describes a specific, sensitive, semiautomated, and quantitative Hep-2 cell culture-based 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay for Bacillus cereus emetic toxin. Of nine Bacillus, Brevibacillus, and Paenibacillus species assessed for emetic toxin production, only B. cereus was cytotoxic.
منابع مشابه
Identification of emetic toxin producing Bacillus cereus strains by a novel molecular assay.
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Of the toxins produced by Bacillus cereus, the emetic toxin is likely the most dangerous but, due to the lack of a suitable assay, the least well known. In this paper, a new, sensitive, inexpensive, and rapid bioassay for detection of the emetic toxin of B. cereus is described. The assay is based on the loss of motility of boar spermatozoa upon 24 h of exposure to extracts of emetic B. cereus s...
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Bacillus cereus is a facultative anaerobic, spore-forming, motile microorganism that can be commonly isolated from food. It is known to produce four toxins, one emetic toxin and three potential diarrheal toxins (Ehling-schulz, et al., 2004; Samapundo, et al., 2011). The emetic syndrome is mainly characterized by vomiting 0.5 6 h after ingestion of the contaminated food. In the diarrheal syndrom...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 65 4 شماره
صفحات -
تاریخ انتشار 1999